橙色熒光蛋白mOrange激發(fā)光譜
mOrange是2004年發(fā)表的一種堿性(組成型熒光)橙色熒光蛋白,來源于Discosoma sp.。它具有高酸敏感性。mOrange is a basic (constitutively fluorescent) orange fluorescent protein published in 2004, derived from Discosoma sp.. It has high acid sensitivity.
morange橙色熒光蛋白的激發(fā)光的中心波長(zhǎng)為548nm,發(fā)射光的中心波長(zhǎng)為562nm。
橙色熒光蛋白mOrange激發(fā)光譜圖:
Orange fluorescent protein Proteins
Orange fluorescent protein (OFP) is a versatile biological marker used to monitor physiological processes, visualize protein localization, and detecting transgenic expression in vivo. OFPSparkTM is a red (orange) fluorescent protein (excitation/emission maxima are 549 and 566 nm, respectively) derived from DsRed. Possessing high photostability and pH stability, OFPSparkTM is more than twice brighter than mOrange2. OFPSparkTM can be expressed and detected in a wide range of organisms.
橙色熒光蛋白 (OFP) 是一種多功能生物標(biāo)記物,用于監(jiān)測(cè)生理過程、可視化蛋白質(zhì)定位和檢測(cè)體內(nèi)轉(zhuǎn)基因表達(dá)。OFPSparkTM 是一種源自 DsRed 的紅色(橙色)熒光蛋白(激發(fā)/發(fā)射zui大值分別為 549 和 566 nm)。OFPSparkTM 具有高光穩(wěn)定性和 pH 穩(wěn)定性,比 mOrange2 亮兩倍以上。OFPSparkTM 可以在多種生物體中表達(dá)和檢測(cè)。
什么是mOrange2熒光蛋白?
mOrange2 fluorescent protein. Living Colors mOrange2 is an extremely bright orange fluorescent protein monomer ideal for use as a tag or reporter. The mOrange2 fluorescent protein mutant derived from a monomeric mutant of DsRed, which was developed in Dr. Roger Tsien’s lab (Nathan C Shaner et al. 2004; Wang et al.
常用熒光蛋白的激發(fā)和發(fā)射光:
熒光蛋白 | 蛋白型號(hào) | 激發(fā)波長(zhǎng)(EX) | 發(fā)射波長(zhǎng)(EM) |
青色熒光蛋白 | AmCyan1 | 458nm | 489nm |
綠色熒光蛋白 Green fluorescent proteins | AcGFP1 | 475nm | 505 nm |
ZsGreen1 | 493nm | 505nm | |
黃色熒光蛋白 Yellow fluorescent proteins | ZsYellow1 | 529nm | 539 nm |
mBanana | 540nm | 553 nm | |
桔色熒光蛋白 Orange fluorescent proteins | mOrange2 | 549nm | 565nm |
紅色熒光蛋白 | DsRed-Express2 | 554nm | 591nm |
DsRed-Express | 554nm | 586nm | |
tdTomato | 554nm | 581 nm | |
DsRed-Monomer | 557nm | 592 nm | |
DsRed2 | 563nm | 582 nm | |
AsRed2 | 576nm | 592 nm | |
mStrawberry | 574nm | 596 nm | |
mCherry | 587nm | 610nm |
EX: Excitation max (nm)
EM: Emission max (nm)
如果您想觀察熒光蛋白在動(dòng)植物上的表達(dá),建議使用便攜式激發(fā)光源,上海峰志儀器有限公司銷售便攜式雙波長(zhǎng)熒光蛋白激發(fā)光源,具體詳細(xì)介紹請(qǐng)瀏覽《便攜式熒光蛋白激發(fā)光源LUYOR-3415RG》。推薦使用LUYOR-3415便攜式雙波長(zhǎng)激發(fā)光源或《LUYOR-3280熒光手電筒》,這樣您就可以用便攜式熒光蛋白激發(fā)光源直接觀察熒光蛋白在植物的種子、愈傷、根系和葉片上的表達(dá)。上海峰志儀器有限公司免費(fèi)提供便攜式熒光蛋白激發(fā)光源樣機(jī)試用,滿意付款,具體請(qǐng)按照網(wǎng)頁底部聯(lián)系信息電話或.聯(lián)系。